Zeiss Plan-Neofluar 40x/1.30 Oil Immersion Phase Contrast Objective PH3 (NA 1.30, Infinity, RMS)

In stock

SKU: 440451

Regular price $2,750.00
Sale price $2,750.00 Regular price $6,200.00 save $3,450.00
The Zeiss Plan-Neofluar 40×/1.30 Oil Immersion Phase Contrast Objective (PH3, RMS) is a high-performance fluorite oil immersion objective with an integrated PH3 phase ring — combining NA 1.30 oil immersion resolution with high-contrast phase contrast imaging capability for living cells and unstained specimens. Oil immersion phase contrast at 40× delivers significantly higher resolution and contrast than equivalent dry phase contrast objectives, making this a premium choice for demanding live-cell imaging workflows. Legacy RMS thread for older Zeiss microscopes. Offered here in excellent condition with flawless optics at significant savings versus the list price.

KEY FEATURES

  • Oil immersion phase contrast — NA 1.30 — the combination of oil immersion and phase contrast at 40× delivers significantly higher resolution than dry phase contrast objectives (typically NA 0.65–0.75), enabling visualization of finer cellular detail in living specimens without staining
  • PH3 phase ring — integrated phase contrast ring compatible with PH3 condenser annuli for high-contrast phase contrast imaging of living cells, bacteria, and unstained specimens
  • Plan-Neofluar fluorite optical design — fluorite glass elements providing excellent chromatic correction and high UV-visible transmission for combined phase contrast and fluorescence imaging
  • RMS thread mount — legacy Zeiss RMS format, compatible with older Zeiss Axioskop, Axiovert, and Axioplan series microscopes with RMS nosepieces
  • 🇩🇪 Zeiss quality, made in Germany — precision optical construction for consistent, high-efficiency light transmission and long service life
  • Condition: Excellent — flawless optics — catalog number 440451
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Product Overview

The Zeiss Plan-Neofluar 40×/1.30 Oil PH3 is a specialized objective combining oil immersion NA with phase contrast capability — a combination that is less common than dry phase contrast objectives but delivers substantially higher resolution for demanding live-cell imaging applications. Standard 40× phase contrast objectives are typically dry with NA 0.65–0.75; the Plan-Neofluar 40×/1.30 Oil PH3 provides NA 1.30 — nearly double the NA of a typical dry 40× phase contrast objective — enabling visualization of finer cellular structures, organelles, and dynamic processes in living cells without fixation or staining. The PH3 phase ring is compatible with PH3 condenser annuli for standard phase contrast imaging. The Plan-Neofluar fluorite design provides excellent chromatic correction and UV-visible transmission for combined phase contrast and fluorescence imaging in the same objective, enabling simultaneous or sequential phase contrast and fluorescence observation of living specimens. This is the legacy RMS thread version (catalog 440451), compatible with older Zeiss Axioskop, Axiovert, and Axioplan microscopes with RMS nosepieces. This unit is offered in excellent condition with flawless optics at a significant discount to the list price of $6,200. Catalog number 440451.

Technical Specifications

Catalog Number 440451
Magnification 40×
Numerical Aperture (NA) 1.30
Immersion Medium Oil
Working Distance 0.21mm
Phase Ring PH3 (for use with PH3 condenser annulus)
Optical Design Plan-Neofluar (fluorite)
Correction Infinity-corrected
Thread / Mount RMS (legacy Zeiss format)
Compatible Microscopes Zeiss Axioskop, Axiovert, Axioplan (RMS nosepiece)
Country of Origin Germany
Condition Excellent — flawless optics
SKU 440451

Microscope Compatibility

Typical Applications

High-resolution phase contrast imaging of living cells at NA 1.30 oil immersion
Combined phase contrast and fluorescence imaging of living specimens
Visualization of fine cellular structures and organelles in unstained living cells
Bacterial and microorganism imaging requiring high-contrast phase contrast at high NA
Live-cell imaging workflows on legacy Zeiss RMS nosepiece microscopes
Any application requiring higher phase contrast resolution than dry 40× objectives provide
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