Zeiss Plan Neofluar 25x Multi-immersion Infinity Corrected Objective
Zeiss Plan Neofluar 25x Multi-immersion Infinity Corrected Objective

Zeiss Plan-Neofluar 25x/0.80 Multi-Immersion Objective (NA 0.80, Oil/Water/Glycerin, Infinity, RMS)

In stock

SKU: 440544

$1,800.00
$1,800.00 $3,800.00 save $2,000.00
The Zeiss Plan-Neofluar 25×/0.80 Multi-Immersion Objective is a specialized live-cell imaging objective supporting three immersion media — oil, water, and glycerin — and specifically designed for use across the physiological temperature range of 23–37°C. This makes it an exceptional choice for live-cell fluorescence imaging in heated stage environments where temperature-induced refractive index changes would degrade image quality with standard objectives. RMS thread with M27 adapter available. Offered here in lightly used condition with flawless optics at significant savings versus the list price.

KEY FEATURES

  • Optimized for 23–37°C live-cell imaging — specifically designed for the physiological temperature range used in live-cell imaging with heated stages and incubation systems, where standard objectives optimized for room temperature can exhibit spherical aberration as temperature rises and refractive indices shift
  • Three immersion media: oil, water, and glycerin — flexibility to match the immersion medium to the specimen type and imaging conditions; water immersion is particularly valuable for live-cell imaging at 37°C where refractive index matching to aqueous specimens is optimal
  • Plan-Neofluar fluorite optical design — fluorite glass elements providing excellent chromatic correction and high UV-visible transmission for multi-channel fluorescence and brightfield imaging
  • NA 0.80 at 25× — good numerical aperture for a multi-immersion objective at 25×, delivering practical resolution and fluorescence light collection for live-cell imaging of single cells and small tissue regions
  • RMS thread + M27 adapter available — fits legacy Zeiss RMS nosepiece microscopes; M27 adapter available upon request for use on current Zeiss Axio series M27 nosepieces
  • 🇩🇪 Zeiss quality, made in Germany — precision optical construction for consistent, high-efficiency light transmission and long service life
  • Condition: Lightly used — flawless optics — catalog number 440544
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Product Overview

The Zeiss Plan-Neofluar 25×/0.80 Multi-Immersion is a purpose-built live-cell imaging objective combining multi-immersion flexibility with temperature-optimized optical design — specifically engineered for the 23–37°C temperature range used in live-cell microscopy with heated stages and incubation systems. Standard objectives are optimized for room temperature (typically 23°C); as temperature rises to 37°C for live-cell imaging, the refractive indices of immersion media and biological specimens shift, introducing spherical aberration that degrades image quality. The Plan-Neofluar 25×/0.80 is designed to maintain optimal optical performance across this temperature range, making it the correct choice for live-cell imaging workflows where image quality at physiological temperature is critical. The multi-immersion design supports oil (n=1.515), water (n=1.333), and glycerin (n≈1.45) — with water immersion being particularly well-suited for live-cell imaging at 37°C, where the refractive index of water closely matches that of aqueous biological specimens and minimizes spherical aberration. At 25×, this objective provides a practical field of view for single-cell and small tissue region imaging, bridging the gap between 20× overview and 40× high-magnification objectives. RMS thread with M27 adapter available upon request. This unit is offered in lightly used condition with flawless optics at a significant discount to the list price of $3,800. Catalog number 440544.

Technical Specifications

Catalog Number 440544
Magnification 25×
Numerical Aperture (NA) 0.80
Immersion Media Oil (n=1.515), Water (n=1.333), Glycerin (n≈1.45)
Working Distance 0.21mm
Temperature Range 23–37°C (optimized for live-cell imaging)
Optical Design Plan-Neofluar (fluorite, multi-immersion)
Correction Infinity-corrected
Thread / Mount RMS (M27 adapter available upon request)
Compatible Microscopes Zeiss Axioskop, Axiovert, Axioplan (RMS); Zeiss Axio series with M27 adapter
Country of Origin Germany
Condition Lightly used — flawless optics
SKU 440544

Microscope Compatibility

Typical Applications

Live-cell fluorescence imaging at 37°C with heated stages and incubation systems
Multi-immersion imaging requiring flexibility between oil, water, and glycerin
Long-term time-lapse imaging of living cells at physiological temperature
Single-cell and small tissue region imaging at 25× bridging 20× and 40×
Multi-channel fluorescence imaging of living specimens requiring temperature-stable optics
Any live-cell imaging workflow where temperature-induced spherical aberration is a concern

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