Zeiss Fluar 5x High Performance Objective M27
SKU: 420130
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ZEISS FLUAR 5X HIGH-TRANSMISSION FLUORESCENCE OBJECTIVE
Maximize fluorescence signal collection and photon efficiency with this specialized high-performance objective, featuring:
- 💡 Optimized for maximum light transmission and photon collection in fluorescence applications
- 🔬 5x magnification with 0.25 numerical aperture for wide-field fluorescence imaging
- 📏 Generous 12.5mm working distance for thick specimens and manipulation
- 🔧 M27 thread mount for Zeiss infinity-corrected microscope systems
- ⭐ Excellent pre-owned condition with flawless optical performance
🔧 Product Description
The Zeiss Fluar 5x objective represents specialized optical engineering for demanding fluorescence microscopy applications where signal intensity and photon collection efficiency are critical. Manufactured from proprietary low-fluorescence glass formulations, Fluar objectives minimize autofluorescence while maximizing transmission across UV, visible, and near-infrared wavelengths. This 5x magnification provides an optimal balance between field of view and resolution for screening fluorescently-labeled specimens, multi-well plate imaging, and large-area tissue section analysis. The 0.25 numerical aperture delivers sufficient light-gathering capability for moderate-intensity fluorescence signals, while the 12.5mm working distance accommodates thick specimens, microfluidic devices, and allows room for environmental chambers or manipulation tools. The M27 thread mount ensures compatibility with Zeiss Axio, Axioskop, and other infinity-corrected platforms. Fluar objectives are particularly valued in quantitative fluorescence applications including calcium imaging, FRET analysis, and multi-color immunofluorescence where consistent photon collection and minimal optical aberrations are essential for accurate measurements.
🔎 Typical Applications
- Quantitative fluorescence microscopy and photometry
- Multi-color immunofluorescence imaging of tissue sections
- Live-cell calcium imaging and ion indicator studies
- FRET (Förster Resonance Energy Transfer) analysis
- High-throughput screening and multi-well plate imaging
- Fluorescence in situ hybridization (FISH) applications
📊 Key Specifications
| Specification | Details |
| Catalog Number | 420130-9900 |
| Magnification | 5x |
| Numerical Aperture (NA) | 0.25 |
| Working Distance | 12.5mm |
| Optical Design | Fluar (high-transmission fluorescence) |
| Correction | Infinity-corrected |
| Thread Mount | M27 (Zeiss standard) |
| Condition | Excellent with flawless optics (pre-owned) |
| Primary Advantage | Maximum photon collection for quantitative fluorescence |
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