Olympus XLFluor 4X/340 Objective – Macro Fluorescence LWD

The Olympus XLFluor 4×/340 Objective is a specialized macro fluorescence lens engineered for low-magnification fluorescence observation of large specimens. With an ultra-long 29.25 mm working distance and high transmission at 340 nm wavelength, this objective is specifically designed for applications requiring UV excitation, large field of view, and exceptional specimen clearance.

The XLFluor-340 series represents Olympus's specialized design for macro fluorescence applications where standard objectives cannot provide adequate working distance or field coverage. The ultra-long 29.25 mm working distance provides exceptional clearance for large specimens such as whole organisms, tissue sections, multi-well plates, and samples requiring environmental chambers or manipulation during observation.

High transmission at 340 nm wavelength makes this objective ideal for UV fluorescence applications, particularly DAPI (4',6-diamidino-2-phenylindole) nuclear staining and other UV-excitable fluorophores. The specialized optical coatings and glass materials ensure efficient transmission of UV light, maximizing fluorescence signal intensity and enabling sensitive detection of weakly fluorescent samples.

At 4× magnification with a numerical aperture of 0.28, this objective provides an excellent balance between field of view and light-gathering capability. The high NA for this magnification ensures bright fluorescence images with good resolution, while the low magnification enables observation of large sample areas — essential for screening applications, whole-organism imaging, and large-area tissue analysis.

The macro fluorescence design enables observation of specimens that are too large for standard microscope objectives. This includes whole-mount embryos, intact organs, plant tissues, multi-well culture plates, and other samples where low magnification and long working distance are essential. The large field of view and exceptional clearance make this objective invaluable for developmental biology, neuroscience, and high-throughput screening applications.

As an infinity-corrected objective, this lens is designed for modern microscope systems where the image is formed at infinity, allowing the insertion of optical components such as filters, dichroic mirrors, and beam splitters in the parallel light path without introducing aberrations. This makes it fully compatible with advanced fluorescence techniques and filter cube configurations.

Featuring an M34 thread mount, this objective is compatible with Olympus BX, CX, and IX series microscope systems equipped for macro observation. The robust construction and precision Japanese manufacturing ensure reliable performance in demanding research environments where low-magnification fluorescence imaging of large specimens is essential. This is a special order item for specialized macro fluorescence applications.