Olympus WI-DICT2 DIC Prism

The Olympus WI-DICT2 is a Nomarski differential interference contrast (DIC) prism designed specifically for use with the Olympus WI-SRE3 and WI-SNPXLU2 water immersion condenser systems. DIC microscopy uses a Nomarski prism pair to split and recombine polarized light beams that travel through slightly different lateral positions in the specimen — converting refractive index and thickness gradients into amplitude contrast. The result is a striking pseudo-three-dimensional relief image that reveals membrane boundaries, organelle structure, and surface topology in transparent, unstained living specimens with exceptional clarity. The WI-DICT2 is engineered for the optical geometry of water immersion condenser configurations, which are commonly used in upright microscope setups for patch-clamp electrophysiology, live brain slice imaging, and other demanding live cell applications where high numerical aperture water immersion objectives and condensers are required. Built to Olympus factory specifications, the WI-DICT2 ensures precise prism alignment and consistent DIC performance.