Olympus WI-DICT DIC Prism

The Olympus WI-DICT is the standard Nomarski differential interference contrast (DIC) prism for water immersion transmitted light microscopy on Olympus upright microscopes. DIC microscopy uses a Nomarski prism to split and recombine polarized light beams traveling through slightly different lateral positions in the specimen — converting refractive index and thickness gradients into amplitude contrast. The result is a striking pseudo-three-dimensional relief image that reveals membrane boundaries, organelle structure, and surface topology in transparent, unstained living specimens with exceptional clarity. The WI-DICT is engineered for water immersion condenser configurations, which are standard in upright microscope setups for patch-clamp electrophysiology, live brain slice imaging, and other demanding live cell applications requiring high numerical aperture water immersion objectives and condensers. Compared to the WI-DICT2 (version 2) and WI-DICTHRA (high resolution variant), the WI-DICT is the original standard prism — a proven, reliable solution for transmitted light DIC in water immersion configurations. This unit is in excellent condition at a significant discount from the list price. Built to Olympus factory specifications (catalog number U-P240), it ensures precise prism alignment and consistent DIC performance.