Olympus WI-DIC25HR DIC Prism

The Olympus WI-DIC25HR is a high resolution DIC condenser prism designed for use with the Olympus XLPLN 25XWMP multiphoton water immersion objective and the WI-UCD universal condenser. In differential interference contrast (DIC) microscopy, the condenser prism acts as a beamsplitter that introduces a precise angular shear between two polarized light wavefronts before they pass through the specimen — the objective-side prism then recombines them, converting optical path differences caused by specimen refractive index and thickness gradients into amplitude contrast. Because DIC prisms must be precisely matched to the numerical aperture and optical geometry of the objective they are paired with, the WI-DIC25HR is specifically engineered for the XLPLN 25XWMP, ensuring optimal shear distance, wavefront matching, and maximum spatial resolution at 25x magnification. The XLPLN 25XWMP is a high numerical aperture, long working distance multiphoton water immersion objective widely used in two-photon and multiphoton imaging, intravital microscopy, and deep tissue imaging — making the WI-DIC25HR the correct DIC condenser prism for systems that combine multiphoton imaging with transmitted light DIC on the same platform. The WI-UCD universal condenser houses multiple DIC prisms in a revolving turret for rapid switching between objective-matched prisms. Built to Olympus factory specifications, the WI-DIC25HR delivers consistent, high-contrast DIC performance at 25x in water immersion configurations.