Olympus UPLANSAPO 40x Silicone Objective

The Olympus UPlanSApo 40× Silicone Objective represents the pinnacle of biological microscopy optics, combining ultra-high numerical aperture, super apochromat correction, and silicone immersion technology to deliver exceptional performance for demanding live cell imaging, deep tissue observation, and advanced fluorescence microscopy applications.

Silicone immersion technology provides superior refractive index matching compared to traditional oil or water immersion. With a refractive index of approximately 1.40, silicone closely matches the refractive index of living cells and tissues, minimizing spherical aberration when imaging deep into thick specimens. This makes silicone immersion ideal for live cell imaging, tissue culture observation, and any application requiring deep penetration into biological samples.

The ultra-high 1.25 numerical aperture delivers exceptional resolution and light-gathering capability, revealing fine cellular structures, organelles, and molecular details with unparalleled clarity. This high NA is particularly valuable for fluorescence microscopy, where maximum light collection efficiency is essential for detecting weak fluorescent signals and achieving optimal signal-to-noise ratios.

Super apochromat correction represents the highest level of chromatic aberration correction available. Unlike standard achromat or semi-apochromat objectives, super apochromats correct for chromatic aberration across the entire visible spectrum and into the near-infrared. This ensures accurate color rendition, eliminates color fringing, and enables multi-color fluorescence imaging with perfect registration between channels — critical for colocalization studies and quantitative imaging.

The Universal Plan (UPlan) optical design provides exceptional field flatness, maintaining sharp focus and consistent illumination from the center to the edge of the field of view. This is essential for photomicrography, image stitching, and quantitative image analysis where uniform image quality across the entire field is required.

As an infinity-corrected objective, this lens is designed for modern microscope systems where the image is formed at infinity, allowing the insertion of optical components such as filters, dichroic mirrors, and beam splitters in the parallel light path without introducing aberrations. This makes it fully compatible with advanced fluorescence techniques including confocal microscopy, multiphoton imaging, and super-resolution microscopy.

Featuring a standard RMS thread mount and a field number of 26.5, this objective is compatible with Olympus BX, CX, and IX series microscope systems. The robust construction and precision Japanese manufacturing ensure reliable performance in demanding research environments where the highest image quality and optical performance are essential.