Olympus UMPLFLN 10X Water Immersion (Dipping) Microscope Objective (NA 0.30, Infinity)

The Olympus UMPLFLN 10XW Water Immersion Objective (N2667500) is a versatile biological objective designed for wide-field imaging of living cells and tissues in aqueous environments. This objective combines low magnification with exceptional working distance, making it ideal for specimen survey, feature location, and imaging large tissue regions or cell populations.

The 10× magnification and 0.30 numerical aperture provide a wide field of view that enables researchers to survey large tissue areas, locate regions of interest, and observe multiple cells or tissue structures simultaneously. This magnification is particularly valuable in neuroscience for imaging entire brain slice regions, in developmental biology for observing whole embryos or large tissue explants, and in cell biology for monitoring cell populations and migration patterns.

The 10mm working distance is exceptional for a water immersion objective, providing generous clearance for accommodating thick specimens, deep tissue imaging, and experimental manipulations. This extended working distance enables imaging deep into brain slices, organotypic cultures, and thick tissue sections while maintaining optical quality. The long working distance is also critical for electrophysiology experiments where microelectrodes, patch pipettes, or stimulation devices must access cells while maintaining optical observation.

The Plan Fluor (UMPLFLN) optical design incorporates fluorite glass elements that provide excellent chromatic aberration correction across the visible spectrum. This correction is essential for fluorescence microscopy where multiple fluorophores with different emission wavelengths are imaged simultaneously. The flat-field (Plan) correction ensures sharp focus across the entire field of view—particularly important at low magnifications where the field is large.

Water immersion objectives offer significant advantages over oil immersion for live-cell imaging and long-term time-lapse experiments. Water is non-toxic to cells, evaporates cleanly without residue, and maintains physiological conditions. The refractive index of water (n=1.33) closely matches that of aqueous biological specimens, minimizing spherical aberration when imaging deep into tissue.

The infinity-corrected optical design is compatible with Olympus BX (upright), CX (compact), and IX (inverted) series microscopes, enabling integration with fluorescence filter cubes, DIC optics, and camera systems for advanced imaging workflows.

The RMS thread mount ensures compatibility with standard Olympus biological microscope nosepieces.