Olympus PlanApo 40x/0.90 W LSM Water Immersion Objective (NA 0.90, WD 1.0mm, Infinity, RMS)

The Olympus PlanApo 40×/0.90 W LSM is a purpose-built objective for laser scanning and multiphoton microscopy — combining water immersion, plan apochromat correction, and a 1.0mm working distance in a package specifically optimized for the demands of confocal and two-photon imaging. The W LSM designation indicates that this objective has been engineered for laser scanning microscopy applications, with optical coatings and glass elements selected for high transmission across the broad spectral range required for two-photon excitation (typically 700–1000nm NIR) as well as the visible spectrum for confocal imaging. Water immersion is the preferred medium for deep tissue imaging because its refractive index (n=1.333) closely matches that of biological cytoplasm (~1.38) and aqueous specimens, minimizing spherical aberration as the focal plane moves deeper into tissue — a critical advantage over oil immersion objectives, which introduce increasing spherical aberration with depth due to refractive index mismatch. The 1.0mm working distance provides practical depth access for imaging thick brain slices, tissue sections, organoids, and other thick biological specimens. The plan apochromat correction ensures accurate chromatic registration across all fluorescence channels for multi-color deep tissue imaging. Compatible with Olympus BX, CX, and IX series microscopes with RMS nosepieces. This unit is offered in excellent condition with flawless optics at a significant discount to the list price of $5,600. SKU PLAPO40XWLSM.