Olympus LUMFLN 60X Water Immersion (Dipping) Microscope Objective (NA 1.10, Infinity)

The Olympus LUMFLN 60X Water Dipping Objective (N2667900) is a premium biological objective designed for deep tissue fluorescence imaging and electrophysiology in living brain slices and tissue preparations. This objective represents the gold standard for neuroscience research, combining ultra-high numerical aperture with long working distance and semi-apochromat optical quality for the most demanding brain slice imaging applications.

The 60× magnification and 1.10 numerical aperture provide exceptional resolution and light-gathering capability, enabling visualization of fine cellular structures, dendritic spines, and subcellular compartments in living neurons. The ultra-high 1.10 NA is among the highest available for water dipping objectives, providing outstanding performance for fluorescence imaging, two-photon microscopy, and IR-DIC applications. This high NA is critical for detecting weak fluorescent signals from genetically encoded indicators, calcium dyes, and voltage-sensitive dyes in living tissue.

The 1.5mm working distance provides clearance for deep tissue penetration while accommodating patch pipettes, stimulation electrodes, and other experimental tools. This working distance enables imaging neurons located 50–200 micrometers deep in brain slices—the typical depth range for healthy, viable neurons in acute slice preparations. The dipping design allows the objective to be lowered directly into the recording chamber, with the front lens element immersed in the physiological saline bathing the tissue.

The correction collar is a precision adjustment mechanism that optimizes optical performance for variations in tissue preparation, chamber depth, and optical path length. This adjustment is particularly valuable in brain slice imaging where tissue thickness and mounting conditions can vary between experiments. Proper correction collar adjustment ensures maximum resolution and contrast by compensating for spherical aberration introduced by optical path variations.

The LUMFLN (Long-distance Universal Medium Fluorite) optical design incorporates semi-apochromat (fluorite) glass elements that provide excellent chromatic aberration correction across a broad wavelength range. This correction is essential for multi-color fluorescence imaging where GFP, YFP, RFP, and other fluorophores are imaged simultaneously, and for IR-DIC imaging combined with visible fluorescence—a common workflow in neuroscience where IR-DIC guides electrode placement while fluorescence identifies genetically labeled cell populations.

This objective is specifically optimized for brain tissue fluorescence and patch clamp work, making it the preferred choice for neuroscience laboratories performing electrophysiology combined with optical imaging. The combination of high NA, long working distance, and excellent optical correction makes this objective ideal for two-photon calcium imaging, optogenetics, dendritic spine imaging, and synaptic physiology experiments.

This objective is compatible with Olympus BX (upright) series microscopes, the standard platform for electrophysiology and brain slice imaging. The RMS thread mount ensures compatibility with standard microscope nosepieces.

This unit is brand new, ready for the most demanding neuroscience and physiology research applications.