Olympus LU PLANAPO 25x Silicone Immersion Microscope Objective (NA 0.85, Infinity)

The Olympus LU PLANAPO 25× Silicone Immersion Objective (NA 0.85) is a super apochromat silicone immersion objective from Olympus’s LU (upright/inverted universal) series — combining a generous 2.0mm working distance with silicone refractive index matching and super apochromat correction for wide-field live-cell fluorescence, deep tissue imaging, and multi-color confocal applications at intermediate magnification.

• 🧫 Silicone immersion (n=1.40) — refractive index closely matches biological cytoplasm and tissue (~1.38), reducing spherical aberration at depth for superior image quality throughout thick specimens compared to water or oil immersion
• 📏 Long working distance: 2.0mm — exceptional clearance for a silicone immersion objective at this NA, enabling imaging of thick specimens, organoids, brain slices, and experimental setups requiring tool access beneath the objective
• 🌟 Super apochromat (PLANAPO) correction — the highest level of chromatic aberration correction, ensuring accurate multi-channel co-localization and sharp imaging across the full visible spectrum
• 📍 Field number 26.5 — large field of view for 25× magnification, enabling wide-area imaging of organoids, embryos, brain slices, and multi-cell preparations
• ♾️ Infinity-corrected, RMS thread — compatible with Olympus BX and IX series microscopes
• 🇯🇵 Olympus quality, made in Japan — precision optical construction for consistent, high-efficiency light transmission and long service life
• ✅ New — part number N6432700

🔧 Product Description

The Olympus LU PLANAPO 25× Silicone is a specialized intermediate-magnification objective that addresses the need for wide-field, high-quality imaging of large biological structures at depth. The 25× magnification with FN 26.5 provides a wide imaging field ideal for organoids, brain slices, embryos, and other large specimens where both field coverage and optical performance at depth are required. The 2.0mm working distance is exceptional for a silicone immersion objective at NA 0.85 — providing ample clearance for thick specimens, imaging chambers, and experimental manipulations beneath the objective. Silicone immersion (n=1.40) closely matches the refractive index of biological cytoplasm and tissue (~1.38), reducing spherical aberration when imaging deep into living specimens and maintaining image quality at depths where water immersion objectives suffer increasing aberration. The super apochromat (PLANAPO) correction ensures accurate chromatic registration across all fluorescence channels, essential for multi-color co-localization and any imaging workflow where chromatic shift between channels would compromise data integrity. Compatible with Olympus BX and IX series microscopes. Supplied new from stock, part number N6432700.

🔎 Typical Applications

• Wide-field live-cell fluorescence imaging of organoids, embryos, and brain slices
• Deep tissue confocal imaging requiring long working distance and refractive index matching
• Multi-color fluorescence co-localization requiring super apochromat correction
• Neuroscience and electrophysiology with simultaneous optical imaging at intermediate magnification
• 3D reconstruction of large biological structures requiring wide field and depth performance
• Long-term time-lapse imaging of thick specimens with minimal spherical aberration drift

📊 Key Specifications

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