Nikon CFI75 Apo 25XC W Water Dipping Microscope Objective | 420–1400 nm

The Nikon CFI75 Apo 25XC W represents the pinnacle of water dipping objective design, engineered specifically for advanced multiphoton microscopy, two-photon imaging, and deep tissue neuroscience applications. As part of Nikon's prestigious CFI75 optical system, this apochromat objective delivers exceptional chromatic correction across an extraordinarily broad wavelength range from 420 nm (visible blue) through 1400 nm (near-infrared), making it ideal for multiphoton excitation, second harmonic generation (SHG), and third harmonic generation (THG) imaging. The ultra-high numerical aperture of 1.1 in water immersion provides outstanding resolution and light-gathering efficiency, enabling visualization of fine cellular structures deep within living tissue. Unlike conventional water immersion objectives requiring coverglass, this dipping objective is designed for direct immersion into physiological buffers, eliminating optical aberrations and enabling imaging deep into brain slices, organotypic cultures, and intact tissue preparations. The 2mm working distance provides ample clearance for micromanipulation equipment while maintaining the high NA essential for multiphoton imaging, and the integrated correction collar allows precise compensation for coverglass thickness variations (0–0.17mm) when needed. The apochromatic optical design ensures minimal chromatic aberration across the entire wavelength range, critical for accurate co-localization in multi-color imaging and quantitative analysis. Compatible with Nikon's advanced upright microscope systems, this objective is an essential tool for laboratories conducting cutting-edge neuroscience research, in vivo imaging, optogenetics, and any application requiring the highest optical performance for deep tissue multiphoton microscopy.