Leica HCX PL APO 63x/1.30 Glycerol Immersion Objective (NA 1.30, Infinity)

Out of stock

SKU: 506193

Regular price $5,500.00
Sale price $5,500.00 Regular price $7,500.00 save $2,000.00
The Leica HCX PL APO 63×/1.30 Glycerol Immersion Objective is a high-performance plan apochromat glycerol immersion objective designed specifically for live-cell and deep tissue fluorescence imaging — using glycerol (n≈1.45) as the immersion medium to closely match the refractive index of living cells and biological tissues, reducing spherical aberration and maintaining image quality at depth. Offered here in excellent condition with flawless optics at significant savings versus the new list price.

KEY FEATURES

  • Glycerol immersion (n≈1.45) — refractive index closely matches living cells and biological tissues (~1.38–1.46), dramatically reducing spherical aberration when imaging deep into live specimens compared to water or oil immersion
  • NA 1.30 at 63× — high numerical aperture for outstanding resolution and fluorescence light collection in a magnification range ideal for single-cell and subcellular imaging
  • Plan apochromat (HCX PL APO) correction — Leica’s highest level of chromatic and spherical aberration correction for accurate multi-channel fluorescence co-localization across the full visible spectrum
  • Working distance: 0.28mm — optimized for standard coverslip imaging with correction collar for refractive index adjustment
  • Infinity-corrected, M25 thread — compatible with Leica DMI, DM, and SP series microscopes
  • 🇩🇪 Leica quality, made in Germany — precision optical construction for consistent, high-efficiency light transmission
  • Condition: Excellent — flawless optics — catalog number 506193
Backed by Spach Optics Warranty

Secure Packaging & Reliable Delivery

People-Powered Support

Product Overview

The Leica HCX PL APO 63×/1.30 Glycerol is a specialized objective engineered for the demands of live-cell and deep tissue fluorescence microscopy where refractive index matching to the specimen is critical. Glycerol immersion (n≈1.45) sits between water (n=1.33) and oil (n=1.515) and closely matches the refractive index of living cells and biological tissues (~1.38–1.46), dramatically reducing spherical aberration when imaging deep into living specimens — maintaining image quality and resolution at depths where water immersion objectives suffer increasing aberration and oil immersion is incompatible with aqueous specimens. The HCX PL APO correction delivers Leica’s highest level of chromatic and spherical aberration correction, ensuring accurate co-localization across all fluorescence channels. At 63× with NA 1.30, this objective provides high-resolution single-cell and subcellular imaging with excellent fluorescence light collection. This unit is offered in excellent condition with flawless optics — optically indistinguishable from new — at a significant discount to the new list price of $7,500. Compatible with Leica DMI, DM, and SP series confocal microscopes with M25 thread nosepieces. Catalog number 506193.

Technical Specifications

Catalog Number 506193
Magnification 63×
Numerical Aperture (NA) 1.30
Immersion Medium Glycerol (n≈1.45)
Working Distance 0.28mm
Optical Design HCX PL APO (Plan Apochromat)
Correction Infinity-corrected
Thread / Mount M25
Compatible Microscopes Leica DMI, DM, SP series
Country of Origin Germany
Condition Excellent — flawless optics
SKU 506193

Microscope Compatibility

Typical Applications

Live-cell fluorescence and confocal imaging requiring refractive index matching to biological tissue
Deep tissue imaging where water immersion suffers spherical aberration at depth
Multi-color fluorescence co-localization requiring plan apochromat correction
Organoid, brain slice, and thick tissue section imaging at high magnification
Long-term time-lapse imaging of living specimens with minimal aberration drift
Any application where oil immersion is incompatible and water immersion is insufficient at depth
  • Designed for Mixed-Brand Lab Environments

    Olympus, Nikon, Zeiss, Leica components in one place

  • Quality-Verified New & Pre-Owned Equipment

    Professionally sourced and verified for performance

  • Backed by Spach Optics Warranty & Support

    Ideal for labs using multiple microscope platforms

  • Fast, Reliable U.S. Shipping

    Most orders ship quickly from within the United States

  • Expert Support from Optical Specialists

    Guidance on compatibility, selection, and configuration