Leica HCX PL APO 40x/1.25 Oil Immersion Objective (NA 1.25, Infinity, M25)

The Leica HCX PL APO 40×/1.25 Oil Immersion Objective is a high-performance plan apochromat oil immersion objective from Leica’s HCX (High Correction) series — delivering NA 1.25 resolution with Leica’s highest-level plan apochromat chromatic and spherical aberration correction for demanding confocal, widefield fluorescence, DIC, and brightfield imaging at 40× on Leica DMI and DM series microscopes. Offered here in light use condition with flawless optics at significant savings versus the list price.

• 🔬 NA 1.25 oil immersion at 40× — high numerical aperture for outstanding resolution and fluorescence light collection at intermediate magnification, ideal for single-cell and subcellular imaging
• 🌟 HCX PL APO (High Correction Plan Apochromat) — Leica’s HCX designation indicates high-correction optics with plan apochromat chromatic and spherical aberration correction for accurate multi-channel fluorescence co-localization and flat-field imaging across the full visible spectrum
• 💡 Optimized for confocal and widefield fluorescence — plan apochromat correction ensures accurate chromatic registration across all fluorescence channels, essential for multi-color co-localization, FRET, and quantitative fluorescence imaging
• 📏 Working distance: 0.22mm — practical clearance for standard #1.5 coverslip imaging with M25 thread mount
• ♾️ Infinity-corrected, M25 thread — compatible with Leica DMI, DM, and SP series microscopes
• 🇩🇪 Leica quality, made in Germany — precision optical construction for consistent, high-efficiency light transmission and long service life
• ✅ Condition: Light use — flawless optics — catalog number 506179

🔧 Product Description

The Leica HCX PL APO 40×/1.25 Oil is a premium plan apochromat objective from Leica’s HCX (High Correction) series — a line that represents Leica’s highest level of optical correction for biological microscopy. The HCX designation indicates that this objective meets Leica’s most stringent correction standards, combining plan apochromat chromatic correction with optimized spherical aberration correction for the highest image quality in confocal, widefield fluorescence, and DIC imaging. At NA 1.25 with oil immersion, this objective provides excellent resolution and fluorescence light collection for single-cell and subcellular imaging — protein localization, organelle dynamics, multi-channel co-localization, and FRET studies. The plan apochromat correction ensures accurate chromatic registration across all fluorescence channels, essential for any imaging workflow where chromatic shift between channels would compromise data integrity. The 40× magnification provides a practical balance between field of view and resolution for a wide range of biological imaging applications. This unit is offered in light use condition with flawless optics at a significant discount to the list price of $4,200. Compatible with Leica DMI, DM, and SP series microscopes with M25 thread nosepieces. Catalog number 506179.

🔎 Typical Applications

• Multi-channel confocal and widefield fluorescence imaging at 40×
• Single-cell and subcellular fluorescence imaging including protein localization and organelle dynamics
• Multi-color co-localization and FRET experiments requiring plan apochromat chromatic accuracy
• DIC imaging of living cells and unstained biological specimens
• Histology and pathology slide examination at 40× with oil immersion
• General-purpose high-NA oil immersion imaging on Leica systems

📊 Key Specifications

University and institutional purchase orders are welcome. Please contact us for invoicing and procurement documentation.