Leica HC PL APO 40x/1.30 Oil Immersion Objective with UV Transmission (NA 1.30, 340nm, Infinity)

The Leica HC PL APO 40×/1.30 Oil UV is a specialized plan apochromat objective that combines the optical performance of Leica’s highest-correction oil immersion objective with UV transmission capability at 340nm — making it one of the few oil immersion objectives capable of supporting fura-2 ratiometric calcium imaging at 40×. Fura-2 requires excitation at 340nm and 380nm, with the ratio of emission intensities used to calculate intracellular calcium concentration. Standard oil immersion objectives have significantly reduced transmission below 380nm, making them unsuitable for accurate fura-2 ratiometric imaging. The HC PL APO UV designation indicates that this objective maintains high transmission at 340nm, ensuring sufficient UV excitation intensity for accurate ratiometric calcium measurements. The plan apochromat correction ensures accurate chromatic registration across all fluorescence channels — essential for multi-color imaging combining UV-excited calcium indicators with visible fluorophores for simultaneous structural and functional imaging. At NA 1.30 with oil immersion, this objective provides excellent fluorescence light collection efficiency, critical for UV fluorescence applications where the photon budget is inherently limited. This unit is offered in excellent condition with flawless optics at a significant discount to the new list price of $4,995. Compatible with Leica DMI, DM, and SP series microscopes with M25 thread nosepieces. Catalog number 506329.