Olympus UPlanSApo 30x Silicone Immersion Microscope Objective (NA 1.05, Infinity)

The Olympus UPlanSApo 30× Silicone Immersion Objective (NA 1.05) is a super apochromat silicone immersion objective designed for advanced live-cell fluorescence and confocal imaging — delivering exceptional chromatic correction and refractive index matching to biological specimens for superior depth performance and reduced spherical aberration in thick, aqueous samples.

• 🧫 Silicone immersion (n=1.40) — refractive index closely matches that of biological cytoplasm and tissue (~1.38), dramatically reducing spherical aberration when imaging deep into living cells and thick specimens compared to water or oil immersion
• 🔬 NA 1.05 at 30× — exceptionally high numerical aperture for this magnification, delivering outstanding resolution and fluorescence light collection in a wide-field format ideal for multi-cell and tissue-level imaging
• 🌟 Super apochromat (UPlanSApo) correction — the highest level of chromatic aberration correction in the Olympus objective lineup, ensuring accurate co-localization across multiple fluorescence channels
• 📍 Field number 26.5 — large field of view for 30× magnification, enabling wide-area imaging of tissue sections, organoids, and multi-cell preparations
• ♾️ Infinity-corrected, RMS thread — compatible with Olympus BX, CX, and IX series microscopes
• 🇯🇵 Olympus quality, made in Japan — precision optical construction for consistent, high-efficiency light transmission and long service life
• ✅ New — part number N4212100

🔧 Product Description

The Olympus UPlanSApo 30× Silicone is a specialized high-NA objective that addresses one of the fundamental challenges in deep-tissue fluorescence microscopy: refractive index mismatch. Standard water immersion objectives (n=1.33) and oil immersion objectives (n=1.515) both suffer from increasing spherical aberration as imaging depth increases in biological specimens, because the refractive index of biological cytoplasm and tissue (~1.38) falls between the two. Silicone immersion oil (n=1.40) closely matches this biological refractive index, dramatically reducing spherical aberration at depth and maintaining image quality and resolution throughout thick specimens — making the UPlanSApo 30× Silicone the preferred choice for deep confocal imaging, 3D reconstruction of thick tissue sections, and long-term live-cell imaging where maintaining optical performance at depth is critical. The super apochromat correction ensures accurate chromatic registration across all fluorescence channels, essential for multi-color co-localization studies. The 30× magnification with NA 1.05 and FN 26.5 provides a wide imaging field at high resolution — ideal for imaging organoids, brain slices, embryos, and other large biological structures where both field coverage and optical performance at depth are required. Compatible with Olympus BX, CX, and IX series microscopes. Supplied new from stock, part number N4212100.

🔎 Typical Applications

• Deep tissue confocal and two-photon microscopy in thick biological specimens
• 3D reconstruction of organoids, brain slices, and whole-mount specimens
• Multi-color fluorescence co-localization requiring super apochromat correction
• Live-cell imaging where refractive index matching to cytoplasm is critical
• Embryo and developmental biology imaging at intermediate magnification
• Long-term time-lapse imaging of thick specimens with minimal spherical aberration drift

📊 Key Specifications

University and institutional purchase orders are welcome. Please contact us for invoicing and procurement documentation.