{"product_id":"zeiss-plan-neofluar-25x-multi-immersion-infinity-corrected-objective-1","title":"Zeiss Plan-Neofluar 25x\/0.80 Multi-Immersion Objective (NA 0.80, Oil\/Water\/Glycerin, Infinity, RMS)","description":"\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003eThe \u003cstrong\u003eZeiss Plan-Neofluar 25×\/0.80 Multi-Immersion Objective\u003c\/strong\u003e is a specialized live-cell imaging objective supporting three immersion media — oil, water, and glycerin — and specifically designed for use across the physiological temperature range of 23–37°C. This makes it an exceptional choice for live-cell fluorescence imaging in heated stage environments where temperature-induced refractive index changes would degrade image quality with standard objectives. RMS thread with M27 adapter available. Offered here in lightly used condition with flawless optics at significant savings versus the list price.\u003c\/p\u003e\n\n\u003cul style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e \n\u003cli\u003e🌡️ \u003cstrong\u003eOptimized for 23–37°C live-cell imaging\u003c\/strong\u003e — specifically designed for the physiological temperature range used in live-cell imaging with heated stages and incubation systems, where standard objectives optimized for room temperature can exhibit spherical aberration as temperature rises and refractive indices shift\u003c\/li\u003e\n\u003cli\u003e💧 \u003cstrong\u003eThree immersion media: oil, water, and glycerin\u003c\/strong\u003e — flexibility to match the immersion medium to the specimen type and imaging conditions; water immersion is particularly valuable for live-cell imaging at 37°C where refractive index matching to aqueous specimens is optimal\u003c\/li\u003e\n\u003cli\u003e🌟 \u003cstrong\u003ePlan-Neofluar fluorite optical design\u003c\/strong\u003e — fluorite glass elements providing excellent chromatic correction and high UV-visible transmission for multi-channel fluorescence and brightfield imaging\u003c\/li\u003e\n\u003cli\u003e🔬 \u003cstrong\u003eNA 0.80 at 25×\u003c\/strong\u003e — good numerical aperture for a multi-immersion objective at 25×, delivering practical resolution and fluorescence light collection for live-cell imaging of single cells and small tissue regions\u003c\/li\u003e\n\u003cli\u003e🔧 \u003cstrong\u003eRMS thread + M27 adapter available\u003c\/strong\u003e — fits legacy Zeiss RMS nosepiece microscopes; M27 adapter available upon request for use on current Zeiss Axio series M27 nosepieces\u003c\/li\u003e\n\u003cli\u003e🇩🇪 \u003cstrong\u003eZeiss quality, made in Germany\u003c\/strong\u003e — precision optical construction for consistent, high-efficiency light transmission and long service life\u003c\/li\u003e\n\u003cli\u003e✅ \u003cstrong\u003eCondition: Lightly used — flawless optics\u003c\/strong\u003e — catalog number 440544\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e🔧 Product Description\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003eThe Zeiss Plan-Neofluar 25×\/0.80 Multi-Immersion is a purpose-built live-cell imaging objective combining multi-immersion flexibility with temperature-optimized optical design — specifically engineered for the 23–37°C temperature range used in live-cell microscopy with heated stages and incubation systems. Standard objectives are optimized for room temperature (typically 23°C); as temperature rises to 37°C for live-cell imaging, the refractive indices of immersion media and biological specimens shift, introducing spherical aberration that degrades image quality. The Plan-Neofluar 25×\/0.80 is designed to maintain optimal optical performance across this temperature range, making it the correct choice for live-cell imaging workflows where image quality at physiological temperature is critical. The multi-immersion design supports oil (n=1.515), water (n=1.333), and glycerin (n≈1.45) — with water immersion being particularly well-suited for live-cell imaging at 37°C, where the refractive index of water closely matches that of aqueous biological specimens and minimizes spherical aberration. At 25×, this objective provides a practical field of view for single-cell and small tissue region imaging, bridging the gap between 20× overview and 40× high-magnification objectives. RMS thread with M27 adapter available upon request. This unit is offered in lightly used condition with flawless optics at a significant discount to the list price of $3,800. Catalog number 440544.\u003c\/p\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e🔎 Typical Applications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e \n\u003cli\u003eLive-cell fluorescence imaging at 37°C with heated stages and incubation systems\u003c\/li\u003e\n\u003cli\u003eMulti-immersion imaging requiring flexibility between oil, water, and glycerin\u003c\/li\u003e\n\u003cli\u003eLong-term time-lapse imaging of living cells at physiological temperature\u003c\/li\u003e\n\u003cli\u003eSingle-cell and small tissue region imaging at 25× bridging 20× and 40×\u003c\/li\u003e\n\u003cli\u003eMulti-channel fluorescence imaging of living specimens requiring temperature-stable optics\u003c\/li\u003e\n\u003cli\u003eAny live-cell imaging workflow where temperature-induced spherical aberration is a concern\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e📊 Key Specifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width:100%; border-collapse: collapse; font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e\n\u003ctr style=\"background-color: #f2f2f2;\"\u003e\n\u003cth style=\"text-align:left; padding: 6px; border: 1px solid #ddd;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"text-align:left; padding: 6px; border: 1px solid #ddd;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCatalog Number\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e440544\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eMagnification\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e25×\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eNumerical Aperture (NA)\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e0.80\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eImmersion Media\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eOil (n=1.515), Water (n=1.333), Glycerin (n≈1.45)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eWorking Distance\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e0.21mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eTemperature Range\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e23–37°C (optimized for live-cell imaging)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eOptical Design\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003ePlan-Neofluar (fluorite, multi-immersion)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCorrection\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eInfinity-corrected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eThread \/ Mount\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eRMS (M27 adapter available upon request)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCompatible Microscopes\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eZeiss Axioskop, Axiovert, Axioplan (RMS); Zeiss Axio series with M27 adapter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCountry of Origin\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eGermany\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCondition\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eLightly used — flawless optics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eSKU\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e440544\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e\u003cem\u003eUniversity and institutional purchase orders are welcome. Please contact us for invoicing and procurement documentation. M27 adapter available upon request — please mention when ordering.\u003c\/em\u003e\u003c\/p\u003e","brand":"Spach Optics, Inc.","offers":[{"title":"Default Title","offer_id":51695636742445,"sku":"440544","price":1800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0933\/7965\/8029\/files\/ZEISS-440544-25x-2.jpg?v=1765532773","url":"https:\/\/www.spachoptics.com\/products\/zeiss-plan-neofluar-25x-multi-immersion-infinity-corrected-objective-1","provider":"Spach Optics, Inc.","version":"1.0","type":"link"}