{"product_id":"olympus-uplanapo-100x-hr-tirf-objective-copy","title":"Olympus UAPO N 100X TIRF Objective 1.49 NA Oil Immersion","description":"\u003cp class=\"MsoNormal\" style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003eOlympus UAPO N 100× OTIRF Apochromat Objective (NA 1.49, Oil Immersion, Infinity)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e🔬 100× magnification, NA 1.49 (oil)\u003cbr\u003e\n🌟 Apochromat optical design with superior chromatic correction\u003cbr\u003e\n💡 TIRF-optimized for evanescent field excitation\u003cbr\u003e\n📏 Working distance: ~0.12 mm\u003cbr\u003e\n🔧 Correction collar included\u003cbr\u003e\n🔧 Standard RMS thread (20.32 × 36 TPI)\u003cbr\u003e\n🆕 New condition\u003cbr\u003e\n🎓 University purchase orders accepted\u003c\/p\u003e\n\u003chr\u003e\n\u003cp class=\"MsoNormal\" style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e🔧 Product Description\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003eThe \u003cstrong\u003eOlympus UAPO N 100× OTIRF objective\u003c\/strong\u003e is engineered for \u003cstrong\u003etotal internal reflection fluorescence (TIRF) microscopy\u003c\/strong\u003e and advanced high-resolution fluorescence imaging. With an \u003cstrong\u003eultra-high numerical aperture of 1.49\u003c\/strong\u003e, this objective enables precise excitation at the glass–sample interface, making it ideal for single-molecule imaging, membrane dynamics, and surface-localized fluorescence studies. The apochromat optical design delivers exceptional chromatic correction, contrast, and signal efficiency across the visible spectrum—essential for demanding live-cell and multi-color fluorescence applications.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003eTIRF microscopy requires ultra-high NA objectives (≥1.45) to generate the critical angle necessary for total internal reflection. The evanescent field produced penetrates only ~100–200 nm beyond the coverslip surface, selectively exciting fluorophores at the cell membrane while eliminating background from the bulk of the sample. The 1.49 NA of this objective maximizes the usable angle range for TIRF, delivering exceptional contrast and signal-to-noise ratio for single-molecule detection and membrane imaging. Lower NA objectives cannot generate a proper evanescent field and result in poor signal and high background.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003eThe \u003cstrong\u003ecorrection collar\u003c\/strong\u003e compensates for optical path variations to maintain optimal image quality across different coverslip thicknesses and mounting conditions. The infinity-corrected optical design is compatible with Olympus BX, CX, and IX series microscopes, with a 180 mm tube lens and field number of 22 mm.\u003c\/p\u003e\n\u003chr\u003e\n\u003cp class=\"MsoNormal\" style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e🔎 Typical Applications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\n\u003cli\u003eTotal internal reflection fluorescence (TIRF) microscopy\u003c\/li\u003e\n\u003cli\u003eSingle-molecule fluorescence imaging and tracking\u003c\/li\u003e\n\u003cli\u003eMembrane dynamics and surface interaction studies\u003c\/li\u003e\n\u003cli\u003eLive-cell fluorescence microscopy\u003c\/li\u003e\n\u003cli\u003eHigh-NA super-resolution workflows (PALM\/STORM)\u003c\/li\u003e\n\u003cli\u003eReceptor internalization and exocytosis studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003cp class=\"MsoNormal\" style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e💧 TIRF vs Standard Fluorescence Objectives\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003eTIRF Objectives (e.g., this objective, NA 1.49)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\n\u003cli\u003eUltra-high NA (1.45–1.70+)\u003c\/li\u003e\n\u003cli\u003eExcite only a thin surface layer (~100–200 nm)\u003c\/li\u003e\n\u003cli\u003eExtremely high contrast and low background\u003c\/li\u003e\n\u003cli\u003eBest for:\n\u003cul\u003e\n\u003cli\u003eMembrane imaging\u003c\/li\u003e\n\u003cli\u003eSingle-molecule detection\u003c\/li\u003e\n\u003cli\u003eLive-cell surface dynamics\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"MsoNormal\" style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003eStandard Fluorescence Objectives\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\n\u003cli\u003eLower NA (0.75–1.40)\u003c\/li\u003e\n\u003cli\u003eIlluminate entire sample depth\u003c\/li\u003e\n\u003cli\u003eMore background fluorescence\u003c\/li\u003e\n\u003cli\u003eBetter for:\n\u003cul\u003e\n\u003cli\u003eGeneral fluorescence imaging\u003c\/li\u003e\n\u003cli\u003eThick samples and tissue sections\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003cp class=\"MsoNormal\" style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e📊 Key Specifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" cellpadding=\"8\" cellspacing=\"0\" style=\"font-family: 'Segoe UI Emoji', sans-serif; border-collapse: collapse; width: 100%;\"\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eDetails\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eModel\u003c\/td\u003e\n\u003ctd\u003eOlympus UAPO N 100× OTIRF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSKU\u003c\/td\u003e\n\u003ctd\u003eUAPON100XOTIRF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnification\u003c\/td\u003e\n\u003ctd\u003e100×\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNumerical Aperture (NA)\u003c\/td\u003e\n\u003ctd\u003e1.49\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptical Correction\u003c\/td\u003e\n\u003ctd\u003eApochromat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eImmersion Medium\u003c\/td\u003e\n\u003ctd\u003eOil\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTIRF Compatible\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorking Distance\u003c\/td\u003e\n\u003ctd\u003e~0.12 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eParfocal Length\u003c\/td\u003e\n\u003ctd\u003e45 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eField Number\u003c\/td\u003e\n\u003ctd\u003e22 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTube Lens\u003c\/td\u003e\n\u003ctd\u003e180 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWavelength Range\u003c\/td\u003e\n\u003ctd\u003eUV–VIS (optimized for fluorescence)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMount\u003c\/td\u003e\n\u003ctd\u003eRMS (20.32 × 36 TPI)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCorrection Collar\u003c\/td\u003e\n\u003ctd\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOptical System\u003c\/td\u003e\n\u003ctd\u003eInfinity-corrected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCondition\u003c\/td\u003e\n\u003ctd\u003eNew\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003chr\u003e\n\u003cp class=\"MsoNormal\" style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cem\u003eUniversity and institutional purchase orders accepted. Expert technical support available. Contact us with any questions before ordering.\u003c\/em\u003e\u003c\/p\u003e","brand":"spachoptics","offers":[{"title":"Default Title","offer_id":51835602829613,"sku":"UAPON100XOTIRF","price":8500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0933\/7965\/8029\/files\/UAPON100XOTIRF1_c4d0aed8-888d-4ae5-9f2c-b875bb527b4b.jpg?v=1771638391","url":"https:\/\/www.spachoptics.com\/products\/olympus-uplanapo-100x-hr-tirf-objective-copy","provider":"Spach Optics, Inc.","version":"1.0","type":"link"}