{"product_id":"olympus-planapo-40x-water-immersion-objective","title":"Olympus PlanApo 40x\/0.90 W LSM Water Immersion Objective (NA 0.90, WD 1.0mm, Infinity, RMS)","description":"\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003eThe \u003cstrong\u003eOlympus PlanApo 40×\/0.90 W LSM Water Immersion Objective\u003c\/strong\u003e is a specialized plan apochromat water immersion objective from Olympus — specifically optimized for laser scanning microscopy (LSM) and two-photon\/multiphoton imaging, combining a 1.0mm working distance with NA 0.90 water immersion and plan apochromat correction for deep tissue fluorescence imaging. The W LSM designation indicates this objective is engineered for the high-intensity pulsed laser illumination and broad spectral transmission requirements of multiphoton microscopy. Offered here in excellent condition with flawless optics at significant savings versus the list price.\u003c\/p\u003e\n\n\u003cul style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e \n\u003cli\u003e🔬 \u003cstrong\u003eOptimized for laser scanning and multiphoton microscopy (W LSM)\u003c\/strong\u003e — the LSM designation indicates this objective is specifically engineered for confocal and two-photon laser scanning microscopy, with optical coatings and glass elements optimized for high-intensity pulsed laser illumination and broad near-infrared transmission required for two-photon excitation\u003c\/li\u003e\n\u003cli\u003e💧 \u003cstrong\u003eWater immersion — ideal refractive index matching for biological tissue\u003c\/strong\u003e — water immersion (n=1.333) closely matches the refractive index of biological cytoplasm (~1.38) and aqueous specimens, minimizing spherical aberration for accurate 3D imaging and deep tissue penetration\u003c\/li\u003e\n\u003cli\u003e📏 \u003cstrong\u003eLong working distance: 1.0mm\u003c\/strong\u003e — generous clearance for deep tissue imaging, enabling penetration into thick tissue sections, brain slices, and organoids well beyond the reach of standard short-working-distance objectives\u003c\/li\u003e\n\u003cli\u003e🌟 \u003cstrong\u003ePlan apochromat correction\u003c\/strong\u003e — Olympus plan apochromat chromatic and spherical aberration correction for accurate multi-channel fluorescence co-localization and flat-field imaging across the full visible and near-infrared spectrum\u003c\/li\u003e\n\u003cli\u003e♾️ \u003cstrong\u003eInfinity-corrected, RMS thread\u003c\/strong\u003e — compatible with Olympus BX, CX, and IX series microscopes with RMS nosepieces\u003c\/li\u003e\n\u003cli\u003e🇯🇵 \u003cstrong\u003eOlympus quality, made in Japan\u003c\/strong\u003e — precision optical construction for consistent, high-efficiency light transmission and long service life\u003c\/li\u003e\n\u003cli\u003e✅ \u003cstrong\u003eCondition: Excellent — flawless optics\u003c\/strong\u003e — SKU PLAPO40XWLSM\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e🔧 Product Description\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003eThe Olympus PlanApo 40×\/0.90 W LSM is a purpose-built objective for laser scanning and multiphoton microscopy — combining water immersion, plan apochromat correction, and a 1.0mm working distance in a package specifically optimized for the demands of confocal and two-photon imaging. The W LSM designation indicates that this objective has been engineered for laser scanning microscopy applications, with optical coatings and glass elements selected for high transmission across the broad spectral range required for two-photon excitation (typically 700–1000nm NIR) as well as the visible spectrum for confocal imaging. Water immersion is the preferred medium for deep tissue imaging because its refractive index (n=1.333) closely matches that of biological cytoplasm (~1.38) and aqueous specimens, minimizing spherical aberration as the focal plane moves deeper into tissue — a critical advantage over oil immersion objectives, which introduce increasing spherical aberration with depth due to refractive index mismatch. The 1.0mm working distance provides practical depth access for imaging thick brain slices, tissue sections, organoids, and other thick biological specimens. The plan apochromat correction ensures accurate chromatic registration across all fluorescence channels for multi-color deep tissue imaging. Compatible with Olympus BX, CX, and IX series microscopes with RMS nosepieces. This unit is offered in excellent condition with flawless optics at a significant discount to the list price of $5,600. SKU PLAPO40XWLSM.\u003c\/p\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e🔎 Typical Applications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e \n\u003cli\u003eTwo-photon and multiphoton deep tissue imaging requiring NIR transmission and water immersion\u003c\/li\u003e\n\u003cli\u003eConfocal laser scanning microscopy of thick tissue sections and brain slices\u003c\/li\u003e\n\u003cli\u003eDeep tissue fluorescence imaging where refractive index matching to aqueous specimens is critical\u003c\/li\u003e\n\u003cli\u003eOrganoid and 3D culture imaging requiring long working distance at 40×\u003c\/li\u003e\n\u003cli\u003eMulti-channel fluorescence imaging of living tissue requiring plan apochromat correction\u003c\/li\u003e\n\u003cli\u003eAny LSM application requiring water immersion NA 0.90 with extended working distance at 40×\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e📊 Key Specifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width:100%; border-collapse: collapse; font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e\n\u003ctr style=\"background-color: #f2f2f2;\"\u003e\n\u003cth style=\"text-align:left; padding: 6px; border: 1px solid #ddd;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"text-align:left; padding: 6px; border: 1px solid #ddd;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eSKU\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003ePLAPO40XWLSM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eMagnification\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e40×\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eNumerical Aperture (NA)\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e0.90\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eImmersion Medium\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eWater (n=1.333)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eWorking Distance\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e1.0mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eOptical Design\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003ePlanApo W LSM (Plan Apochromat, Water, Laser Scanning)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eSpectral Optimization\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eVisible + NIR (optimized for two-photon\/multiphoton excitation)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCorrection\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eInfinity-corrected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eThread \/ Mount\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eRMS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCompatible Microscopes\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eOlympus BX, CX, IX series (RMS nosepiece)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCountry of Origin\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eJapan\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCondition\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eExcellent — flawless optics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e\u003cem\u003eUniversity and institutional purchase orders are welcome. Please contact us for invoicing and procurement documentation.\u003c\/em\u003e\u003c\/p\u003e","brand":"Spach Optics, Inc.","offers":[{"title":"Default Title","offer_id":51695372796205,"sku":"PLAPO40XWLSM","price":2500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0933\/7965\/8029\/files\/OLYMPUS-40X-WLSM-2.jpg?v=1765519659","url":"https:\/\/www.spachoptics.com\/products\/olympus-planapo-40x-water-immersion-objective","provider":"Spach Optics, Inc.","version":"1.0","type":"link"}