{"product_id":"nikon-cfi-s-fluor-40x-oil-objective","title":"Nikon CFI Super Fluor 40x\/1.30 Oil Immersion Objective (NA 1.30, UV to 340nm, Infinity, M25)","description":"\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003eThe \u003cstrong\u003eNikon CFI Super Fluor 40×\/1.30 Oil Immersion Objective\u003c\/strong\u003e is a UV-optimized high-performance oil immersion objective from Nikon’s CFI Super Fluor series — specifically engineered for superior UV transmission down to 340nm, enabling fura-2 and indo-1 ratiometric calcium imaging, fluo-3 fluorescence, photo-activation of caged compounds, and other UV fluorescence applications where standard oil immersion objectives have insufficient UV transmission. Offered here in excellent condition with flawless optics at significant savings versus the list price.\u003c\/p\u003e\n\n\u003cul style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e \n\u003cli\u003e🔆 \u003cstrong\u003eSuperior UV transmission to 340nm\u003c\/strong\u003e — the defining feature of the CFI Super Fluor series; optimized for maximum light transmission at 340nm and below, enabling fura-2 (340\/380nm), indo-1 (340nm), and photo-activation of caged compounds requiring deep UV excitation\u003c\/li\u003e\n\u003cli\u003e🔬 \u003cstrong\u003eNA 1.30 oil immersion at 40×\u003c\/strong\u003e — high numerical aperture for outstanding resolution and fluorescence light collection, maximizing signal from UV-excited fluorophores where photon budget is critical\u003c\/li\u003e\n\u003cli\u003e🧪 \u003cstrong\u003eOptimized for UV calcium indicators\u003c\/strong\u003e — specifically designed for fura-2 ratiometric calcium imaging (340\/380nm excitation), indo-1 calcium imaging (340nm), and fluo-3 fluorescence in live-cell physiology workflows\u003c\/li\u003e\n\u003cli\u003e💡 \u003cstrong\u003ePhoto-activation of caged compounds\u003c\/strong\u003e — high UV transmission enables efficient photo-uncaging of caged ATP, caged calcium, caged neurotransmitters, and other UV-activated compounds for optogenetics and cell physiology\u003c\/li\u003e\n\u003cli\u003e♾️ \u003cstrong\u003eInfinity-corrected, M25 thread\u003c\/strong\u003e — compatible with Nikon CFI series microscopes including Eclipse Ti2, Ti, Ni, E, and FN series\u003c\/li\u003e\n\u003cli\u003e🇯🇵 \u003cstrong\u003eNikon quality, made in Japan\u003c\/strong\u003e — precision optical construction for consistent, high-efficiency UV and visible light transmission\u003c\/li\u003e\n\u003cli\u003e✅ \u003cstrong\u003eCondition: Excellent — flawless optics\u003c\/strong\u003e — catalog number MRF01401\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e🔧 Product Description\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003eThe Nikon CFI Super Fluor 40×\/1.30 Oil is a purpose-built UV-optimized objective designed for the most demanding UV fluorescence applications in live-cell physiology and cell biology. The Super Fluor designation indicates superior UV transmission down to 340nm — the excitation wavelength of fura-2 and indo-1, the most widely used ratiometric calcium indicators. Standard oil immersion objectives have significantly reduced transmission below 380nm, making them unsuitable for accurate fura-2 ratiometric imaging where the 340nm excitation beam must reach the specimen with sufficient intensity for accurate ratiometric calcium measurements. The CFI Super Fluor series addresses this by using optical glass with high UV transmission, ensuring reliable performance at 340nm and below. Beyond calcium imaging, the high UV transmission also enables efficient photo-activation of caged compounds — caged ATP, caged calcium, caged neurotransmitters, and other UV-activated probes used in cell physiology and optogenetics research — where the efficiency of photo-uncaging depends directly on the UV transmission of the objective. At NA 1.30 with oil immersion, this objective provides excellent fluorescence light collection efficiency, critical for UV fluorescence applications where the photon budget is inherently limited. Compatible with Nikon CFI series microscopes. Catalog number MRF01401.\u003c\/p\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e🔎 Typical Applications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e \n\u003cli\u003eFura-2 ratiometric calcium imaging (340\/380nm excitation) at 40× with oil immersion\u003c\/li\u003e\n\u003cli\u003eIndo-1 calcium imaging requiring UV transmission at 340nm\u003c\/li\u003e\n\u003cli\u003eFluo-3 and other UV-excited fluorescence calcium indicators\u003c\/li\u003e\n\u003cli\u003ePhoto-activation of caged compounds: caged ATP, caged calcium, caged neurotransmitters\u003c\/li\u003e\n\u003cli\u003eMulti-channel fluorescence combining UV calcium indicators with visible fluorophores\u003c\/li\u003e\n\u003cli\u003eAny UV fluorescence application requiring oil immersion NA and superior 340nm transmission\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e📊 Key Specifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width:100%; border-collapse: collapse; font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e\n\u003ctr style=\"background-color: #f2f2f2;\"\u003e\n\u003cth style=\"text-align:left; padding: 6px; border: 1px solid #ddd;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"text-align:left; padding: 6px; border: 1px solid #ddd;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCatalog Number\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eMRF01401\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eMagnification\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e40×\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eNumerical Aperture (NA)\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e1.30\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eImmersion Medium\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eOil\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eWorking Distance\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e0.2mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eUV Transmission\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eSuperior transmission to 340nm (fura-2, indo-1, caged compounds)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eOptical Design\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCFI Super Fluor (UV-optimized fluorite)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCorrection\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eInfinity-corrected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eThread \/ Mount\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eM25\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCompatible Microscopes\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eNikon CFI Eclipse Ti2, Ti, Ni, E, FN series\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCountry of Origin\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eJapan\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCondition\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eExcellent — flawless optics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eSKU\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eMRF01401\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e\u003cem\u003eUniversity and institutional purchase orders are welcome. Please contact us for invoicing and procurement documentation.\u003c\/em\u003e\u003c\/p\u003e","brand":"Spach Optics, Inc.","offers":[{"title":"Default Title","offer_id":51695344812333,"sku":"MRF01401","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0933\/7965\/8029\/files\/NIKON-MRF01401-2.gif?v=1765516887","url":"https:\/\/www.spachoptics.com\/products\/nikon-cfi-s-fluor-40x-oil-objective","provider":"Spach Optics, Inc.","version":"1.0","type":"link"}