{"product_id":"leica-hcx-pl-apo-63x-glycerol-immersion-objective","title":"Leica HCX PL APO 63x\/1.30 Glycerol Immersion Objective (NA 1.30, Infinity)","description":"\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003eThe \u003cstrong\u003eLeica HCX PL APO 63×\/1.30 Glycerol Immersion Objective\u003c\/strong\u003e is a high-performance plan apochromat glycerol immersion objective designed specifically for live-cell and deep tissue fluorescence imaging — using glycerol (n≈1.45) as the immersion medium to closely match the refractive index of living cells and biological tissues, reducing spherical aberration and maintaining image quality at depth. Offered here in excellent condition with flawless optics at significant savings versus the new list price.\u003c\/p\u003e\n\n\u003cul style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e \n\u003cli\u003e🧪 \u003cstrong\u003eGlycerol immersion (n≈1.45)\u003c\/strong\u003e — refractive index closely matches living cells and biological tissues (~1.38–1.46), dramatically reducing spherical aberration when imaging deep into live specimens compared to water or oil immersion\u003c\/li\u003e\n\u003cli\u003e🔬 \u003cstrong\u003eNA 1.30 at 63×\u003c\/strong\u003e — high numerical aperture for outstanding resolution and fluorescence light collection in a magnification range ideal for single-cell and subcellular imaging\u003c\/li\u003e\n\u003cli\u003e🌟 \u003cstrong\u003ePlan apochromat (HCX PL APO) correction\u003c\/strong\u003e — Leica’s highest level of chromatic and spherical aberration correction for accurate multi-channel fluorescence co-localization across the full visible spectrum\u003c\/li\u003e\n\u003cli\u003e📏 \u003cstrong\u003eWorking distance: 0.28mm\u003c\/strong\u003e — optimized for standard coverslip imaging with correction collar for refractive index adjustment\u003c\/li\u003e\n\u003cli\u003e♾️ \u003cstrong\u003eInfinity-corrected, M25 thread\u003c\/strong\u003e — compatible with Leica DMI, DM, and SP series microscopes\u003c\/li\u003e\n\u003cli\u003e🇩🇪 \u003cstrong\u003eLeica quality, made in Germany\u003c\/strong\u003e — precision optical construction for consistent, high-efficiency light transmission\u003c\/li\u003e\n\u003cli\u003e✅ \u003cstrong\u003eCondition: Excellent — flawless optics\u003c\/strong\u003e — catalog number 506193\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e🔧 Product Description\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003eThe Leica HCX PL APO 63×\/1.30 Glycerol is a specialized objective engineered for the demands of live-cell and deep tissue fluorescence microscopy where refractive index matching to the specimen is critical. Glycerol immersion (n≈1.45) sits between water (n=1.33) and oil (n=1.515) and closely matches the refractive index of living cells and biological tissues (~1.38–1.46), dramatically reducing spherical aberration when imaging deep into living specimens — maintaining image quality and resolution at depths where water immersion objectives suffer increasing aberration and oil immersion is incompatible with aqueous specimens. The HCX PL APO correction delivers Leica’s highest level of chromatic and spherical aberration correction, ensuring accurate co-localization across all fluorescence channels. At 63× with NA 1.30, this objective provides high-resolution single-cell and subcellular imaging with excellent fluorescence light collection. This unit is offered in excellent condition with flawless optics — optically indistinguishable from new — at a significant discount to the new list price of $7,500. Compatible with Leica DMI, DM, and SP series confocal microscopes with M25 thread nosepieces. Catalog number 506193.\u003c\/p\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e🔎 Typical Applications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e \n\u003cli\u003eLive-cell fluorescence and confocal imaging requiring refractive index matching to biological tissue\u003c\/li\u003e\n\u003cli\u003eDeep tissue imaging where water immersion suffers spherical aberration at depth\u003c\/li\u003e\n\u003cli\u003eMulti-color fluorescence co-localization requiring plan apochromat correction\u003c\/li\u003e\n\u003cli\u003eOrganoid, brain slice, and thick tissue section imaging at high magnification\u003c\/li\u003e\n\u003cli\u003eLong-term time-lapse imaging of living specimens with minimal aberration drift\u003c\/li\u003e\n\u003cli\u003eAny application where oil immersion is incompatible and water immersion is insufficient at depth\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif;\"\u003e\u003cstrong\u003e📊 Key Specifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width:100%; border-collapse: collapse; font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e\n\u003ctr style=\"background-color: #f2f2f2;\"\u003e\n\u003cth style=\"text-align:left; padding: 6px; border: 1px solid #ddd;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"text-align:left; padding: 6px; border: 1px solid #ddd;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCatalog Number\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e506193\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eMagnification\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e63×\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eNumerical Aperture (NA)\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e1.30\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eImmersion Medium\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eGlycerol (n≈1.45)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eWorking Distance\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e0.28mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eOptical Design\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eHCX PL APO (Plan Apochromat)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCorrection\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eInfinity-corrected\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eThread \/ Mount\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eM25\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCompatible Microscopes\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eLeica DMI, DM, SP series\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCountry of Origin\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eGermany\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eCondition\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eExcellent — flawless optics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003eSKU\u003c\/td\u003e\n\u003ctd style=\"padding: 6px; border: 1px solid #ddd;\"\u003e506193\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003chr\u003e\n\n\u003cp style=\"font-family: 'Segoe UI Emoji', sans-serif; color: rgb(0,0,0);\"\u003e\u003cem\u003eUniversity and institutional purchase orders are welcome. Please contact us for invoicing and procurement documentation.\u003c\/em\u003e\u003c\/p\u003e","brand":"Spach Optics, Inc.","offers":[{"title":"Default Title","offer_id":51695299363117,"sku":"506193","price":5500.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0933\/7965\/8029\/files\/LEICA-63X-GLYCEROL-2.jpg?v=1765512193","url":"https:\/\/www.spachoptics.com\/products\/leica-hcx-pl-apo-63x-glycerol-immersion-objective","provider":"Spach Optics, Inc.","version":"1.0","type":"link"}